Long‐range PCR allows sequencing of mitochondrial genomes from environmental DNA
نویسندگان
چکیده
منابع مشابه
Multiplexed DNA Sequence Capture of Mitochondrial Genomes Using PCR Products
BACKGROUND To utilize the power of high-throughput sequencers, target enrichment methods have been developed. The majority of these require reagents and equipment that are only available from commercial vendors and are not suitable for the targets that are a few kilobases in length. METHODOLOGY/PRINCIPAL FINDINGS We describe a novel and economical method in which custom made long-range PCR pr...
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Recent improvements in next-generation sequencing (NGS) technologies can facilitate the obtainment of mitochondrial genomes. However, it is not clear whether NGS could be effectively used to reconstruct the mitogenome with high gene rearrangement. These high rearrangements would cause amplification failure, and/or assembly and alignment errors. Here, we choose two frogs with rearranged gene ord...
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Alternatives for sequencing of PCR products essentially fall into one of two categories; generation of single-stranded DNA for sequencing or the direct sequencing of double-stranded product. Of the two alternatives, sequencing of double-stranded PCR products is likely to be of greatest immediate significance in terms of general applicability and rapidity. Double-stranded sequencing allows the u...
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ژورنال
عنوان ژورنال: Methods in Ecology and Evolution
سال: 2017
ISSN: 2041-210X,2041-210X
DOI: 10.1111/2041-210x.12836